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CRISPR/CAS9 in human cell culture

November 9 @ 13:00 - 15:00

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DNA manipulation has been a breakthrough in the scientific community enabling a better understanding of the relationship between the genome and its functions. One of the most exciting systems to perform DNA manipulations is Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas). With this system genes could be e. g. added, removed, and even nucleotides can be manipulated. In this workshop, the participants will learn how CRISPR technology has fueled a genome editing revolution and how one can use it in practice by:
– strategies to find the appropriate target region
– searching for candidate guide RNAs (gRNAs) for Cas9 or deadCas9 (dCas9) using different online tools
– molecular tools to achieve a CRISPR/Cas9 mediated gene knockout or a gene activation or gene repression by second generation CRISPR/Cas9 systems (CRISPRa/CRISPRi)
– learning how cutting-edge systems could still be improved

Overview

Details

Date:
November 9
Time:
13:00 - 15:00
Event Category:

Organizer

AG Brandes
Website:
http://www.vrc.uni-frankfurt.de

Venue

Haus 25B, R31
Theodor-Stern-Kai 7
Frankfurt, 60590 Deutschland
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