CPI Academy Workshops | Registration Desk
Please register online for the workshops on the corresponding workshop page. If you cannot attend, please unregister early enough!
If you have any questions, please contact Dr. Claudia Koch.
Get started with CRISPR in cell culture
June 14 @ 10:30 - 12:30
Beitreten 6 Andere!
Already registered? Click here to unregister
DNA manipulation has been a breakthrough in the scientific community enabling a better understanding of the relationship between the genome and its functions. One of the most exciting systems to perform DNA manipulations is Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas). With this system genes could be e. g. added, removed, and even nucleotides can be manipulated. In this workshop, the participants will learn how CRISPR technology has fueled a genome editing revolution and how one can use it in practice by:
– strategies to find the appropriate target region
– searching for candidate guide RNAs (gRNAs) for Cas9 or deadCas9 (dCas9) using different online tools
– molecular tools to achieve a CRISPR/Cas9 mediated gene knockout or a gene activation or gene repression by second generation CRISPR/Cas9 systems (CRISPRa/CRISPRi)
– learning how cutting-edge systems could still be improved