CPI Academy Workshops | Registration Desk
Analysis of intracellular cAMP signaling
November 10 @ 9:00 - 17:00
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Extracellular chemical substances (first messengers) cannot enter cells directly; however translate physical and chemical signals into second messengers within the cells via cell surface receptors. Cyclic adenosine 3′,5′-monophosphate (cAMP) was the first identified second messenger. Cyclic adenosine monophosphate (cAMP) in mammalian cells is a key second messenger of signal transduction mediated by G-protein coupled receptors (GPCRs). By regulating the activity of downstream effectors such as protein kinase A (PKA), the GTP-exchange protein EPAC and the cyclic-nucleotide-gated ion channels, the intracellular
concentration of cAMP plays a vital role in cellular metabolism and is broadly relevant to pharmacological mechanisms. In this session, the role of cAMP in different tissues will be briefly discussed and then we will learn how to determine cAMP concentration within cells after using different stimuli. An analysis of the results and a discussion will follow.